「生命の謎に迫る　ミトコンドリア細胞」

生命の進化の謎に迫る　ミトコンドリア細胞の論文を完成しました。
オープンアクセスジャーナルなのでだれでも見ることができます。

https://www.oatext.com/pdf/IMM-4-267.pdf

Integrative Molecular Medicine
Research Article ISSN: 2056-6360
Proliferation without nuclei suggests mitochondrial cells (MitoCells) to be prokaryotic nature
Nakano K1,2*, Nakayama T1,3, Shirato Y1, Nakayama N1, Tachikawa E1, Sasaki K1, Tarashima M1, Saito K1 and Osawa M1
1Department of Pediatrics, Tokyo Women’s Medical University
2Nakano Children’s Clinic
3Department of Pediatrics, Ibaraki Prefectural University of Health Sciences

Abstract
It is widely believed that evolutionarily advanced cells require nuclei rich in DNA to proliferate, while mitochondria isolated from nuclei cannot proliferate. We have developed a novel stable cell line, designated “mitochondrial cells (MitoCells)”, maintaining active mitochondria, the majority of which lacked nuclei. MitoCells can be continuously generated in culture. Herein, we report proliferation studies of MitoCells. We found that the nuclear DNA-less (nDNA-less) MitoCells, which were sorted with flow cytometry and were filtrated with 1.2 μmeter and 3 μmeter isopore membrane filters, could exist and proliferate. We also confirmed that the sorted nDNA-less MitoCells had mitochondrial DNA(mtDNA) with T9176C mutation analysis and that the unsorted MitoCells had SURF-1 gene of nDNA. These results suggest that MitoCells could have prokaryotic nature with both mtDNA and nDNA.