https:\/\/www.oatext.com\/pdf\/IMM-4-267.pdf<\/a><\/p>\nIntegrative Molecular Medicine
\nResearch Article ISSN: 2056-6360
\nProliferation without nuclei suggests mitochondrial cells (MitoCells) to be prokaryotic nature
\nNakano K1,2*, Nakayama T1,3, Shirato Y1, Nakayama N1, Tachikawa E1, Sasaki K1, Tarashima M1, Saito K1 and Osawa M1
\n1Department of Pediatrics, Tokyo Women’s Medical University
\n2Nakano Children’s Clinic
\n3Department of Pediatrics, Ibaraki Prefectural University of Health Sciences <\/p>\n
Abstract
\nIt is widely believed that evolutionarily advanced cells require nuclei rich in DNA to proliferate, while mitochondria isolated from nuclei cannot proliferate. We have developed a novel stable cell line, designated \u201cmitochondrial cells (MitoCells)\u201d, maintaining active mitochondria, the majority of which lacked nuclei. MitoCells can be continuously generated in culture. Herein, we report proliferation studies of MitoCells. We found that the nuclear DNA-less (nDNA-less) MitoCells, which were sorted with flow cytometry and were filtrated with 1.2 \u03bcmeter and 3 \u03bcmeter isopore membrane filters, could exist and proliferate. We also confirmed that the sorted nDNA-less MitoCells had mitochondrial DNA(mtDNA) with T9176C mutation analysis and that the unsorted MitoCells had SURF-1 gene of nDNA. These results suggest that MitoCells could have prokaryotic nature with both mtDNA and nDNA. <\/p>\n","protected":false},"excerpt":{"rendered":"
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